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- SHCシリーズ - 使用説明書 (239KB)
- SHC002 - Bulletin (226KB)
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SHC002
Sigma
MISSION® Non-Target shRNA Control Vector
| Synonym: | MISSION® Control Vectors |
Description
| Application | To see more application data, protocols, vector maps visit sigma.com/shrna. |
| General description | The MISSION Non-Target shRNA Control Vector is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target human and mouse genes, making it useful as a negative control in experiments using the MISSION shRNA library clones. The short-hairpin sequence contains 4 base pair mismatches to any known human or mouse gene. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl. |
| Legal Information | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense. |
| MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| WGK Germany | 1 |
Related Products
| Recommended | SHC001V, MISSION® pLKO.1-puro Control Transduction Particles |
| SHC003V, MISSION® Control Transduction Particles | |
| SHC002V, MISSION® Non-Target shRNA Control Transduction Particles | |
| SHC003, MISSION® Control Vector Purified DNA TurboGFP™ | |
| SHM01, MISSION® ExpressMag® Super Magnetic Kit - Enhance your lentiviral transduction! | |
| SHC004, MISSION® TurboGFP™ shRNA Control Vector | |
| SHC004V, MISSION® TurboGFP™ shRNA Control Transduction Particles | |
| SHM02, MISSION® ExpressMag® 96-Well Magnetic Kit - Enhance your lentiviral transduction in 96-well plate format! |
